Decreased S Phase and G2/M Phase Cells by a Bioactive Fraction from the Hexane Extract of Rhododendron aroboreum Leading to the Apoptosis of HeLa Cells In-Vitro

Search for novel anticancer compounds from natural sources is a priority goal of cancer biologists. Rhododendron arboreum one the most stately and impressive species. In hilly areas, flowers R. are used treatment chronic diseases. The current study was aimed to evaluate anti-proliferative property on HeLa cells. Crude extract flower prepared using serial extraction procedure with different solvents. Cytotoxicity extracts HeLa, CHO human lymphocytes were assessed MTT assay trypan blue dye exclusion methods. further purified by TLC characterized LC-MS method. hexane showed significant cytotoxicity This fractionated thin layer chromatography, 6 fractions isolated, out which fraction 3 maximum cytotoxic activity an IC50 15 μg/mL. mechanism action observed as apoptosis induction indicated elevated caspase inhibition cell cycle reduced population in S G2/M phases. analysis this promising bioactive resulted molecular ion fragment m/z value 437.48 corresponding that brefeldin A, compound. It can be concluded has could due presence warrants studies purification characterization towards development highly efficient therapeutics cancer.
 HIGHLIGHTS
 
 Anti-cancer drug discovery like medicinal herbs having diverse metabolites therapeutic significance provide lead molecules development
 anti-proliferative/apoptotic potential aroboreum, analysed lines MTT, Lactate dehydrogenase, Caspase studies. compound through preparative methods
 potent apoptotic aroboreum demonstrated S-phase phase cells, increased caspase, LDH activities our results
 Brefeldin previously reported microbe, 1st time analysis
 GRAPHICAL ABSTRACT